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(Solved) (Latest ver. Aug 2020) - Intracellularly recording from pyramidal cells in a mouse

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I am recording intracellularly from the neocortex of a mouse. If I place my electrode on one set of pyramidal neurons (Layer 2) and add fenfluramine (a serotonin uptake blocker), whithin 5 minutes the neurons produce GREATER amount of action potentials. But when I place fenfluramine while recording from another set of pyramidal cells (Layer 5 for example), the neurons showed a DECREASE in AP's (action potentials) after 5 minutes of treatment. How is this possible for one drug to affect similar cells in completely different methods?

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This question was answered on: Sep 16, 2020

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