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(Solved) (Latest ver. Aug 2020) - Absorption Spectra for Fluorescent Proteins

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What is the difference between an absorption spectrum, excitation spectrum, and emissions spectrum?

I deduce the identity of two fluorescent protein (label FP3 and FP6) based on absorption spectrum.

The results of the absorption spectra were plotted in two ways: Y-axis as true absorbance or Y-axis as normalized absorbance. How do the two spectra differ? What information is lost by plotting the Y-axis as normalized absorbance and not true absorbance.

Please help me understand the absorption spectra that I got for my two fluorescent protein.

Please see attached for more detail.

 







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