I deduce the identity of two fluorescent protein (label FP3 and FP6) based on absorption spectrum.
The results of the absorption spectra were plotted in two ways: Y-axis as true absorbance or Y-axis as normalized absorbance. How do the two spectra differ? What information is lost by plotting the Y-axis as normalized absorbance and not true absorbance.
Please help me understand the absorption spectra that I got for my two fluorescent protein.
Please see attached for more detail.
This question was answered on: Sep 16, 2020
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