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(Solved) (Latest ver. Aug 2020) - Isolation and culturing of olfactory sensory neurons

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I am writing a research proposal in which I am attempted to describe an experimental procedure to isolate olfactory sensory neurons (OSNs) and analyze/compare the amino acid sequences of olfactory receptors expressed by individual OSNs. To do this, I am proposing isolating individual OSNs of interest, extracting RNA, and conducting reverse transcription to generate cDNA for PCR and sequencing.

The problem I am having is I am not sure if a single OSN is adequate for RNA extraction and reverse transcription. If not, I would need to describe a method for isolating and culturing individual OSNs to produce enough clones for RNA extraction. If a single cell is not enough, what is a way that clones could be generated from the single OSN?


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This question was answered on: Sep 16, 2020

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