Original posting as formulated by the Student:
I am writing a research proposal in which I am attempted to describe an experimental procedure to isolate olfactory sensory neurons (OSNs) and analyze/compare the amino acid sequences of olfactory receptors expressed by individual OSNs. To do this, I am proposing isolating individual OSNs of interest, extracting RNA, and conducting reverse transcription to generate cDNA for PCR and sequencing.
The problem I am having is I am not sure if a single OSN is adequate for RNA extraction and reverse transcription. If not, I would need to describe a method for isolating and culturing individual OSNs to produce enough clones for RNA extraction. If a single cell is not enough, what is a way that clones could be generated from the single OSN?
This question was answered on: Sep 16, 2020
Need a similar solution fast, written anew from scratch? Place your own custom order
We have top-notch tutors who can help you with your essay at a reasonable cost and then you can simply use that essay as a template to build your own arguments. This we believe is a better way of understanding a problem and makes use of the efficiency of time of the student. New solution orders are original solutions and precise to your writing instruction requirements. Place a New Order using the button below.