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(Solved) (Latest ver. Aug 2020) - Net Charge of a Protein and Temperature

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The table below indicates approximate values for these at 25C. For reference, the delta H(ionization) for Tris is 47kJ/mole.

Groupdeta H (ionization)(kJ/mole)
Asp3.9
Glu0
Lys48
Arg48
His28
N-term40
C- term0

How will the net charge of a typical protein change upon lowering the temperature from 37C to 4C, based on this information?

What can be done experimentally to compensate for this?

 







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