The table below indicates approximate values for these at 25C. For reference, the delta H(ionization) for Tris is 47kJ/mole.
Groupdeta H (ionization)(kJ/mole)
How will the net charge of a typical protein change upon lowering the temperature from 37C to 4C, based on this information?
What can be done experimentally to compensate for this?
This question was answered on: Sep 16, 2020
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